姓 名

魏东芝

性 别

男

出生年月

1963

学 历

博士

职称职务

二级教授，鲁华生物技术研究所所长，十大网赌软件学术委员会主任

【奖励荣誉】

上海市重点学科-生物工程学科带头人，国家重点学科-生物化工学科带头人。中国酶工程专业委员会副主任委员， 中国生物工程学会理事，工业和环境生物技术专业委员会委员，上海生物工程学会常务理事，上海生物化学与分子生物学学会常务理事，上海生物医药行业协会理事，中－韩生物技术指导委员会委员，上海市青年科技启明星联谊会理事。魏东芝教授曾获得国家和上海市的多项荣誉，其中包括：教育部新世纪人才、教育部跨世纪优秀人才、教育部优秀青年教师、上海市科技英才提名、上海市青年科技启明星和启明星后、上海市优秀青年教师，享受国务院特殊津贴和上海市特殊津贴，霍英东基金获得者等。

【研究方向】

生物催化工程和生物制药工程。其中，生物催化工程主要包括新生物催化剂的获得，酶的体外进化及酶功能的开发，酶或细胞的固定化技术及生物催化过程工程研究，特别注重基因工程菌及其酶的应用，将基因工程与生物催化工程结合起来，开展水相及非水相生物催化过程研究，包括酶促转酯、酯合成、寡糖合成及手性合成/拆分。生物制药工程主要包括蛋白/多肽药物、酶等基因的克隆与高效表达；基因工程菌的稳定性、培养与发酵过程工程研究；生物技术药物规模化制备平台技术的研究等。

【研究成果】

1 Guoxiu Zhang, Pei Liu, Lei Zhang, Wei Wei,Xuedong Wang, Dongzhi Wei, Wei Wang, Bioprospecting metagenomics of a microbial community on cotton degradation: Mining for new glycoside hydrolases, Journal of Biotechnology, 2016 234: 35-42

2 Huan Zhang,Lulu Shi,Jinping Lin,Ming Sun,Dongzhi Wei,Effective improvement of the activity of membrane-bound alcohol dehydrogenase by overexpression of adhS in Gluconobacter oxydans, Biotechnology Letters, 2016,38, 7:1131-1138.

3 Wenyuan Gao, Haiyang Fan, Lifeng Chen,Hualei Wang, Dongzhi Wei, Efficient kinetic resolution of secondary alcohols using an organic solvent-tolerant esterase in non-aqueous medium, Biotechnology Letters 2016,38 ,7 : 1165-1171.

4 Liang Min, Lei Zhang,Xin Jiang,Wei Wei,Dongzhi Wei, Wei Wang, A New Strategy for the Enzymatic Degradation of Sudan Dyes in Non-alkaline Conditions, Water Air And Soil Pollution 2016 227 6

5 Yuanyuan Yang, Jinping Lin, Dongzhi Wei, Heterologous Overexpression and Biochemical Characterization of a Nitroreductase from Gluconobacter oxydans 621H,Molecular Biotechnology, 2016 58 6: 428-440

6 Senwen Deng, Xiaoqiang Ma, Ming Sun, Dongzhi Wei, Erzheng Su, Efficient enzymatic synthesis of ampicillin using mutant Penicillin G acylase with bio-based solvent glycerol, Catalysis Communications, 2016 79: 31-34

7 Yujie Chen, Dong Yi, Shuiqin Jiang,Dongzhi Wei,Identification of novel thermostable taurine-pyruvate transaminase from Geobacillus thermodenitrificans for chiral amine synthesis,APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 2016 100 7: 3101-3111

8 Nan Liu, Meng Yang, Xiangzhao Mao, Bozhong Mu, Dongzhi Wei, Molecular cloning and expression of a new -neoagarobiose hydrolase from Agarivorans gilvus WH0801 and enzymatic production of 3,6-anhydro-l-galactose, Biotechnology and Applied Biochemistry, 2016 63 2 : 230-237

9 Huihui Sun, Wenyuan Gao, Hualei Wang, Dongzhi Wei, Expression, characterization of a novel nitrilase PpL19 from Pseudomonas psychrotolerans with S-selectivity toward mandelonitrile present in active inclusion bodies, Biotechnology Letters 2016 38 3: 455-461

10 Kai Wu, Lifeng Chen, Haiyang Fan, Zhiqiang Zhao, Hualei Wang, Dongzhi Wei, Synthesis of enantiopure epoxide by one pot chemoenzymatic approach using a highly enantioselective dehydrogenase, Tetrahedron Letters 2016 57 8 : 899-904

11 Wenyuan Gao , Kai Wu, Lifeng Chen , Haiyang Fan, Zhiqiang Zhao, Bei Gao,Hualei Wang, Dongzhi Wei, A novel esterase from a marine mud metagenomic library for biocatalytic synthesis of short-chain flavor esters, Microbial Cell Factories 2016 15: 41

12 Senwen Deng, Xiaoqiang Ma , Erzheng Su, Dongzhi Wei, Efficient cascade synthesis of ampicillin from penicillin G potassium salt using wild and mutant penicillin G acylase from Alcaligenes faecalis, Journal of Biotechnology 2016 219: 142-148

13 Lei Zhang,Xihua Zhao, Guoxiu Zhang, Jiajia Zhang, Xuedong Wang, Suping Zhang, Wei Wang, Dongzhi Wei, Light-inducible genetic engineering and control of non-homologous end-joining in industrial eukaryotic microorganisms: LML 3.0 and OFN 1.0  Scientific Reports 2016 6: 20761

14 Shan Li, Yushu Ma, Dongzhi Wei, Identification of an interaction between EI and a histidine kinase-response regulator hybrid protein in Gluconobacter oxydans, Biochemical and Biophysical Research Communications 2016 470 2: 331-335

15 Jian Deng, Zhiqiang Yao, Kangling Chen, Y. Adam Yuan, Jinping Lin, Dongzhi Wei, Towards the computational design and engineering of enzyme enantioselectivity: A case study by a carbonyl reductase from Gluconobacter oxydans,Journal of Biotechnology, 2016 217: 31-40

16 Jinmao Zhang, Xinyi Tao, Jianwen Liu, Dongzhi Wei, Yuhong Ren, Fe3+-induced bioinspired chitosan hydrogels for the sustained and controlled release of doxorubicin, RSC Advances 2016 6 53: 47940-47947

17 Bei Gao, Youzhi Mao, Lujia Zhang, Lei He, Dongzhi Wei,A novel saccharifying alpha-amylase of Antarctic psychrotolerant fungi Geomyces pannorum: Gene cloning, functional expression, and characterization, STARCH-STARKE 2016 68 1-2: 20-28

18 Shihui Shen, Yushu Ma, Yuhong Ren, Dongzhi Wei, Construction of an Oscillator Gene Circuit by Negative and Positive Feedbacks, Journal of Microbiology and Biotechnology 2016 26 1: 139-144

19 Lei Zhang, Suping Zhang, Xin Jiang,Wei, Wei, Wei Wang, Dongzhi Wei, A novel host-vector system for heterologous protein co-expression and purification in the Trichoderma reesei industrial strain RUT-C30 Biotechnology Letters 2016 38 1: 89-96

20 Jian Zhang, Aiyou Sun, Rui Xu, Xinyi Tao, Yuguo Dong, Xinxin Lv, Dongzhi Wei, Cell-penetrating and endoplasmic reticulum-locating TAT-IL-24-KDEL fusion protein induces tumor apoptosis,Journal of Cellular Physiology 2016 231 1: 84-93

21 Jian Deng，Zhiqiang Yao，Kangling Chen，Y. Adam Yuan，Jinping Lina*，Dongzhi Wei(*)，Towards the computational design and engineering of enzymeenantioselectivity: A case study by a carbonyl reductase from Gluconobacteroxydans，Journal of Biotechnology，2016，217：31-40。

22 Xin Gao, Chengcheng Zhao, Ting Yu, Shengli Yang, Yuhong Ren,Dongzhi Wei*, Construction of a reusable multi-enzyme supramolecular device via disulfide bond locking. Chemical Communications, 51(2015):10131-10133.

23 Dongbing Cui, Lujia Zhang(*), Shuiqin Jiang, Zhiqiang Yao, Bei Gao, Jingpin Lin, Y Adam Yuan, Dongzhi Wei(*). A computational strategy for altering an enzyme in its cofactor preference to NAD(H) and/or NADP(H), FEBS JOURNAL, 2015, 2339-2351

24 Chen, R., Deng, J., Lin, J., Yin, X., Xie, T., Yang, S., & Wei, D. Assessing the stereoselectivity of carbonyl reductases toward the reduction of OPBE and docking analysis[J]. Biotechnology and applied biochemistry, 2015.

25 Wu K, Wang H, Sun H, Wei D. Efficient kinetic resolution of phenyl glycidyl ether by a novel epoxide hydrolase from Tsukamurella paurometabola[J]. Applied microbiology and biotechnology, 2015, 99(22): 9511-9521.

26 Zhu S, Ma X, Su E, Wei D. Efficient hydration of 2-amino-2, 3-dimethylbutyronitrile to 2-amino-2, 3-dimethylbutyramide in a biphasic system via an easily prepared whole-cell biocatalyst[J]. Green Chemistry, 2015, 17(7): 3992-3999.

27 Hualei Wang; Haiyang Fan; Huihui Sun; Li Zhao; Dongzhi Wei. Process Development for the Production of (R)-(−)-Mandelic Acid by Recombinant Escherichia coli Cells Harboring Nitrilase from Burkholderia cenocepacia J2315[J]. Organic Process Research & Development, 2015, 19(12): 2012-2016.

28 Xingxing Zhang; Baoqi Zhang; Jinping Lin; Dongzhi Wei. Oxidation of ethylene glycol to glycolaldehyde using a highly selective alcohol dehydrogenase from Gluconobacter oxydans[J]. Journal of Molecular Catalysis B: Enzymatic, 2015, 112: 69-75.

29 Rong Chen; Xu Liu; Jiale Wang; Jinping Lin; Dongzhi Wei . Cloning, expression, and characterization of an anti-Prelog stereospecific carbonyl reductase from Gluconobacter oxydans DSM2343[J]. Enzyme and microbial technology, 2015, 70: 18-27.

30 Ting Yu; Xin Gao; Yuhong Ren; Dongzhi Wei. Assembly of cellulases with synthetic protein scaffolds in vitro[J]. Bioresources and Bioprocessing, 2015, 2(1): 1-7.

31 Zhongwei Guo; Kefeng Ni; Dongzhi Wei; Yuhong Ren. Fe 3+-induced oxidation and coordination cross-linking in catechol–chitosan hydrogels under acidic pH conditions[J]. RSC Advances, 2015, 5(47): 37377-37384.

32 Jian Deng; Kangling Chen; Zhiqiang Yao; Jinping Lin; Dongzhi Wei. Efficient synthesis of optically active halogenated aryl alcohols at high substrate load using a recombinant carbonyl reductase from Gluconobacter oxydans[J]. Journal of Molecular Catalysis B: Enzymatic, 2015, 118: 1-7.

33 Xiaoqiang M; Senwen Denga; Erzheng Sub; Dongzhi Wei. One-pot enzymatic production of deacetyl-7-aminocephalosporanic acid from cephalosporin C via immobilized cephalosporin C acylase and deacetylase[J]. Biochemical Engineering Journal, 2015, 95: 1-8.

34 Jianbing Yang; Kefeng Ni; Dongzhi Wei; Yuhong Ren. One-step purification and immobilization of his-tagged protein via Ni2+-functionalized Fe3O4@ polydopamine magnetic nanoparticles[J]. Biotechnology and Bioprocess Engineering, 2015, 20(5): 901-907.

35 Dongshuang Wang; Kefeng Ni; Yuhong Ren; Dongzhi Wei. Site-directed modification of genetically engineered Proteus sp. lipase K107 variants with a polyethylene glycol derivative[J]. Journal of Molecular Catalysis B: Enzymatic, 2015, 111: 36-42.

36 Min Chen; Xiao Lei; Chuze Chen; Shiyang Zhang; Jingli Xie; Dongzhi Wei. Cloning, overexpression, and characterization of a highly active endoinulinase gene from Aspergillus fumigatus Cl1 for production of inulo-oligosaccharides[J]. Applied biochemistry and biotechnology, 2015, 175(2): 1153-1167.

37 Yuguo Dong; Rui Xu; Lihua Wang; Jian Zhang; Chaogang Bai; Aiyou Sun; Dongzhi Wei. A combined feeding strategy for enhancing mycophenolic acid production by fed-batch fermentation in Penicillium brevicompactum[J]. Process Biochemistry, 2015, 50(3): 336-340.

38 Youzhi Mao; Yanchen Yin; Lujia Zhang; Siti Aisyah Alias; Bei Gao; Dongzhi Wei. Development of a novel Aspergillus uracil deficient expression system and its application in expressing a cold-adapted α-amylase gene from Antarctic fungi Geomyces pannorum[J]. Process Biochemistry, 2015, 50(10): 1581-1590.

39 Ying Yang; Lujia Zhang; Mingrong Guo; Jiaqi Sun; Shingo Matsukawa; Jingli Xie; Dongzhi Wei. Novel α-l-Arabinofuranosidase from Cellulomonas fimi ATCC 484 and Its Substrate-Specificity Analysis with the Aid of Computer[J]. Journal of agricultural and food chemistry, 2015, 63(14): 3725-3733.

40 Chao Chen; Yumei Wang; Chun Su; Xinqing Zhao; Ming Li; Xiaowei Meng; Yingyu Jin; Seung-Hwan Yang; Yushu Ma; Dongzhi Wei; Joo-Won Suh. Antifungal activity of Streptomyces albidoflavus L131 against the leaf mold pathogen Passalora fulva involves membrane leakage and oxidative damage[J]. Journal of the Korean Society for Applied Biological Chemistry, 2015, 58(1): 111-119.

41 Bo Yin; Jian Deng; Lirong Lim; Y. Adam Yuan; Dongzhi Wei. Structural insights into stereospecific reduction of α, β-unsaturated carbonyl substrates by old yellow enzyme from Gluconobacter oxydans[J]. Bioscience, biotechnology, and biochemistry, 2015, 79(3): 410-421.

42 Sun, Huihui; Wang, Hualei; Gao, Wenyuan; Chen, Lifeng; Wu, Kai; Wei, Dongzhi. Directed evolution of nitrilase PpL19 from Pseudomonas psychrotolerans L19 and identification of enantiocomplementary mutants toward mandelonitrile[J]. Biochemical and biophysical research communications, 2015, 468(4): 820-825.

43 Wang H; Gao W; Sun H; Chen L; Zhang L; Wang X; Wei D. Protein Engineering of a Nitrilase from Burkholderia cenocepacia J2315 for Efficient and Enantioselective Production of (R)-o-Chloromandelic Acid[J]. Applied and environmental microbiology, 2015, 81(24): 8469-8477.

44 Xin Gao, Shuai Yang, Chengcheng Zhao, Yuhong Ren*, Dong-Zhi Wei*，Artificial multienzyme supramolecular device: highlyordered self-assembly of oligomeric enzymes in vitro and in vivo，Angewandte Chemie，2014，126（51）：14251-14254.

45 Yao, Kang，Xu, Li-Qin，Wang, Feng-Qing*，Wei, Dong-Zhi*，Characterization and engineering of 3-ketosteroid-Delta(1)-dehydrogenase and 3-ketosteroid-9 alpha-hydroxylase in Mycobacterium neoaurum ATCC 25795 to produce 9 alpha-hydroxy-4-androstene-3,17-dione through the catabolism of sterols，Metabolic Engineering，2014，24：181-191.

46 Zhang, Lujia，Tang, Xiaomang，Cui, Dongbing，Yao, Zhiqiang，Gao, Bei，Jiang, Shuiqin，Yin, Bo，Yuan, Y. Adam，Wei, Dongzhi*， A method to rationally increase protein stability based on the charge-charge interaction, with application to lipase LipK107，Protein Science，2014，23（1）：110-116.

47 Yin, Bo，Cui, Dongbing，Zhang, Lujia，Jiang, Shuiqin，Machida, Satoru，Yuan, Y. Adam*，Wei, Dongzhi*，Structural insights into substrate and coenzyme preference by SDR family protein Gox2253 from Gluconobater oxydans，Proteins: Structure, Function, and Genetics，2014，82（11）：2925-2935.

48 Liu, Xu，Wang, Chao，Zhang, Lujia，Yao, Zhiqiang，Cui, Dongbing，Wu, Liang，Lin, Jinping*，Yuan, Yu-Ren Adam，Wei, Dongzhi*，Structural and mutational studies on an aldo-keto reductase AKR5C3 from Gluconobacter oxydans，Protein Science，2014，23（11）：1540-1549.

49 Gao, Xin，Ni, Kefeng，Zhao, Chengcheng，Ren, Yuhong*，Wei, Dongzhi*，Enhancement of the activity of enzyme immobilized on polydopamine-coated iron oxide nanoparticles by rational orientation of formate dehydrogenase，Journal of Biotechnology，2014，188：36-41.

50 You PY, Qiu J, Su EZ, Wei DZ*, Carica papaya Lipase Catalysed Resolution of β-Amino Esters for the Highly Enantioselective Synthesis of (S)-Dapoxetine, EUROPEAN JOURNAL OF ORGANIC CHEMISTRY, 2013, 3: 557-565.

51 Yao K, Wang FQ, Zhang HC, Wei DZ*. Identi?cation and engineering of cholesterol oxidases involved in the initial step of sterols catabolism in Mycobacterium neoaurum. METABOLIC ENGINEERING, 2013,15:75–87.

52 Ni K, Zhou X, Zhao L, Wang HL, Ren YH*, Wei DZ*, Magnetic Catechol-Chitosan with Bioinspired Adhesive Surface: Preparation and Immobilization of v-Transaminase, PLoS ONE, 2012, 7(7) :e41101.

53 Zhang C, Fan K, Zhang W, Zhu R, Zhang L, Wei DZ*. Structure-based characterization of canine-human chimeric uricases and its evolutionary implications. BIOCHIMIE. 2012,94 (6):1412-1420.

54 Liu X, Yuan Z, Yuan Y, Lin JP, Wei DZ*. Biochemical and structural analysis of Gox2181, a new member of the SDR superfamily from Gluconobacter oxydans. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. 2011,415(2): 410-415.

55  Wang JQ, Tao XY, Zhang YF, Wei DZ* ,Ren YH* et al. Reversion of multidrug resistance by tumor targeted delivery of antisense oligodeoxynucleotides in hydroxypropyl-chitosan nanoparticles. BIOMATERIALS 31(15):4426-4433 2010   

56  Wang FQ, Zhang CG, Li B, Wei DZ, Tong WY*, New Microbiological Transformations of Steroids by Streptomyces virginiae IBL-14, ENVIRONMENTAL SCIENCE & TECHNOLOGY 43(15):5967-5974 2009  

57  Ren YH, Wang Y, Zhang YF, Wei DZ* et al, Overcoming multidrug resistance in human carcinoma cells by an antisense oligodeoxynucleotide-doxorubicin conjugate in vitro and in vivo, Conference Information: 7th International Symposium on Polymer Therapeutics, MAY 26-28, 2007 Ctr Invest Principe Felipe, Valencia, SPAIN, MOLECULAR PHARMACEUTICS  5(4) :579-587 2008  

近期代表性专利

1.发明名称：一种微生物细胞转化法生产2-酮基-D-葡萄糖酸的方法，专利号：201310598049.7

2.发明名称：一种耐热葡萄糖淀粉酶及其编码基因与应用，专利号：ZL201310016408.3

3.发明名称：一种用于在里氏木霉细胞内表达外源蛋白的表达设备及其基因工程菌，专利号：201110177811.5

4.发明名称：3-甾酮-Δ1-脱氢酶和工程菌及应用，专利号： ZL201110024534.4

5.发明名称：胆固醇氧化酶基因和工程菌及应用，专利号：ZL201110024406.X